A Quick and Safe Method for Fungal DNA Extraction

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

a simple, inexpensive and safe method for dna extraction of frigid and clotted blood samples

background: extraction of blood genomicdnais one of the main approaches for clinical and molecular biology studies. although several methods have been developed for extraction of blood genomic dna, most of these methods consume long time and use expensive chemicals such as proteinase k and toxic organic solvent such as phenol and chloroform. the objective of this study was to developed easy and...

متن کامل

A Simple and Rapid Leaf Genomic DNA Extraction Method for Polymerase Chain Reaction Analysis

In plants, secondary metabolites and polysaccharides interfere with genomic isolation procedures and downstream reactions such as restriction enzyme analysis and gene amplification. The removal of such contaminants needs complicated and time-consuming protocols. In this study, a simple, rapid and efficient method for leaf DNA extraction was optimized. This method use small amount of plant mater...

متن کامل

An Efficient Method For DNA Extraction From Paraffin Wax Embedded Tissues For PCR Amplification Of Human And Viral DNA

  Background and Objective: Formalin-fixed paraffin-embedded tissues are a valuable source of DNA for molecular studies. We designed and optimized an efficient procedure for DNA extraction from formalin-fixed paraffin embedded tissues. Materials and Methods: Seventy three blocks of cervical paraffin-embedded tissues were investigated. DNA was extracted using 45 minutes boiling in alkaline sol...

متن کامل

A simple method of genomic DNA extraction suitable for analysis of bulk fungal strains.

AIMS A simple and rapid method (designated thermolysis) for extracting genomic DNA from bulk fungal strains was described. METHODS AND RESULTS In the thermolysis method, a few mycelia or yeast cells were first rinsed with pure water to remove potential PCR inhibitors and then incubated in a lysis buffer at 85 degrees C to break down cell walls and membranes. This method was used to extract ge...

متن کامل

Rapid and Efficient Method for the Extraction of Fungal and Oomycetes Genomic DNA

An improved protocol for the isolation of high-quality DNA from fungi is described. The method involves inactivating proteins by SDS/proteinase K and precipitating polysaccharides in the presence of high salt. Further purification is based on differential solubility of DNA and high molecular weight polysaccharides in aqueous media. The procedure does not use the toxic and potentially hazardous ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: The Plant Pathology Journal

سال: 2009

ISSN: 1598-2254

DOI: 10.5423/ppj.2009.25.1.108